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. 2021 Jan 12;11:740. doi: 10.1038/s41598-020-80227-y

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Antimicrobial activities of IDD-B40 and control drugs, including isoniazid (INH) and rifampicin (RIF), against M. tuberculosis H37Rv (A,C,E) and extensively drug-resistant (XDR)-M. tuberculosis (B,D,F). Mycobacterial susceptibility was assessed by resazurin assay (A,B) as RFU/ml (relative fluorescence unit per ml), by microbial cell viability assay (C,D) as RLU/ml (relative luminescence unit per ml), and by CFU enumeration assay (E,F) as CFU/ml. These experiments were carried out in triplicate. Data are given as mean values and standard deviations. *Statistical significance were determined in Graph Pad Prism 8.2.0 between drug treated versus drug-free control using an unpaired Student’s t test (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001).