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. 2021 Jan 12;11:603. doi: 10.1038/s41598-020-79727-8

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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CpepSfGFP profiles in islets in culture. (a) CpepSfGFP islets in culture in collagen with intensity-based “Fire” pseudocoloring (scale on top). (b) Integrated density of the unstimulated individual CpepSfGFP islets over 45 min of culture. (c) Integrated density of CpepSfGFP individual islets in culture during a GSIS in low (1 mM) glucose, then high (26 mM) glucose, low glucose again and finally KCl (30 mM). (c) Individual islets from figure (c) represented as a percentage change from t15 (time at which the islets are stimulated with glucose). Scale bar 500 μm.