Table 2.
List of gene primers used for tissue specific expression pattern of selected immune related genes in C. catla.
| Primers no | Sequence (5′ → 3′) | Annealing temperature (°C) | PCR amplicon size (bp) | Accession |
|---|---|---|---|---|
| β-actin | F: ACCCACACTGTGCCCATCTACG | 60 | 146 | JQ991014 |
| R: ATTTCCCTCTCGGCTGTGGTGG | ||||
| IL-1β | F: ACCCCACAAAACATCGGCCAACC | 60 | 156 | AM932525 |
| R: TCTTCTCCATTTCCACCCTCTC | ||||
| IL-10 | F: CGCAGTGCAGAAGAGTCGAC | 64 | 310 | GU256643 |
| R: CCCGCTTGAGATCCTGAAATAT | ||||
| IFN-γ | F: AAGGGTTCCTGCTCTTGTCA | 54 | 210 | KF590042 |
| R: GCCATTTTTCACCTCGACTG | ||||
| MHC-I | F:AGGAGATGCCGAATGGAG | 56 | 256 | MG859931 |
| R: GATGATTCCCAGCACCAG | ||||
| MyD88 | F: CTTCCAGTTTGTGCATGAGA | 51 | 146 | JN247432 |
| R: CCATCCTCTTGCACCTTTTT | ||||
| NF-kB | F: TTTACAGGAGCGGCGGATAC | 59 | 453 | KY089040 |
| R: GTGCGAAACACGATAGCCAC | ||||
| TNF-α | F: CCAGGCTTTCACTTCAGG | 56 | 181 | FN543477 |
| R: GCCATAGGAATCGGAGTAG | ||||
| TRAF6 | F: CAGTTGACAATGAGGTGCTG | 53 | 328 | MF766465 |
| R: CACACTGTATTGGCGAAAGG | ||||
| TLR2 | F: GACGGTCATGGATGGTTCTTCTTTA | 58 | 131 | HQ293022 |
| R: CAAGATTGCGTATGTAGGCCGTATG | ||||
| TLR3 | F: GCTCCACAGGGTTGAAGACA | 53 | 310 | MF766464 |
| R: GCACGGCCAAGCTTTAGAAT | ||||
| TLR4 | F: ATGATGGAGCGCAATGCCAA | 55 | 140 | GU248418 |
| R: ATGTTACTCAAAGGGTCTCTGCTCC | ||||
| TLR5 | F: CAGGGTAAACATTTCACGCTTCT | 58 | 162 | GU230763 |
| R: ACGCTTTGCCATGGGAACTTT | ||||
| LYZ-C | F: GCTGTGATGTTGTTCGTATCTTC | 66 | 343 | JQ230329 |
| R: GACAGCTTACGCCCATTACAG | ||||
| LYZ-G | F: CATGGGACAGTGAGGAACATC | 66 | 204 | JQ230328 |
| R: CATGTGCTCATATGTACGGACG | ||||
| NOD1 | F: GTTGGTGGGAAATACCTTGCC | 56 | 217 | KC542884 |
| R: TGCTTTCGCCAGACTTCTTCC | ||||
| NOD2 | F: GGCGGGACAGGACGTTTCTCC | 60 | 261 | KC542885 |
| R: GCGGCAACTGAAGGGGAATA |
IL-1β/10 interleukins1β/10, IFN-γ interferon-gamma, TNF-α tumor necrosis factor-alpha, TRAF6 TNF receptor-associated factor 6, TLR2/3/4/5 toll-like receptors 2/3/4/5, LYZ-C/G lysozyme-C/G, NOD1/2 nucleotide binding oligomerization domain containing 1/2, NF-kB nuclear factor kappa-light-chain-enhancer of activated B cells, MyD88 myeloid differentiation primary response 88, MHC-I major histocompatibility complex-class I.