Figure 1.
Anti-proliferative and migration effects of kahweol acetate and cafestol on renal cancer cells. (a–d) ACHN (a,c) and Caki-1 (b,d) cells were seeded in 12-well plates (5 × 104 cells/well) with RPMI containing 10% FBS. Each cell was treated with or without pre-determined concentrations of kahweol acetate and cafestol for 24 and 48 h. (e,f) Anti-proliferative effects of the combination of kahweol acetate and cafestol on ACHN (e) and Caki-1 (f) cells for 24 and 48 h. (g,h) Anti-migration effect of the combination of kahweol acetate and cafestol on ACHN (g) and Caki-1 (h) cells for 12 h. Bar = 500 µm. All experiments were performed in triplicate. Data are presented as means ± standard error of the mean. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. #Synergistic effects were observed.