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. 2021 Jan 12;11:675. doi: 10.1038/s41598-020-80302-4

Figure 4.

Figure 4

The combination of 30 µM kahweol acetate and 30 µM cafestol inhibited Akt and ERK signaling in renal cancer cells. (a–d) Phosphorylation of Akt in ACHN (a) and Caki-1 (b) cells, phosphorylation of ERK in ACHN (c) and Caki-1 (d) cells, at the indicated times after combination treatment were assessed using Western blot analysis and quantitatively analyzed using densitometry with ImageJ software as shown by bar graphs. Equal amounts of cell lysates (10 µg) were subjected to electrophoresis. Data are presented as means ± standard error of the mean (n = 3). *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.