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. 2021 Jan 12;11:675. doi: 10.1038/s41598-020-80302-4

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Kahweol acetate and cafestol treatment promoted the downregulation of immune signaling molecules in renal cancer cells. (a–d) Protein levels of C–C chemokine receptors (CCRs; CCR2/5/6) in ACHN (a) and Caki-1 (b) cells, and programmed death-ligand 1 (PD-L1) in ACHN (c) and Caki-1 (d) cells treated for 24 h were measured using Western blot analysis and quantitatively analyzed using densitometry with ImageJ software as shown by bar graphs. The soluble cell lysates (10 µg in CCR2/5 and PD-L1, 20 µg in CCR6) were subjected to electrophoresis. Data were presented as means ± standard error of the mean (n = 3). *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.