Figure 3: PV plaque size is reduced in cells lacking RACK1.

(A) Analysis of PV plaque numbers. The number of visible poliovirus plaques does not differ between wildtype, RACK1 KO #1 and RACK1-FLAG cell lines. Cells were infected at identical MOI; 42h post infection, cells were stained with crystal violet and poliovirus plaques were counted. Single data points of at least three independent experiments were plotted. The box indicates the data between the interquartile range (IQR) between the 25^th and 75^th percentile, the median is indicated by the thick line within the box. The thin vertical bars represent the 95th percentiles. (B) PV plaques in RACK1 KO #1 cells are smaller compared to PV plaques in wildtype and RACK1-FLAG addback cells. Cells were infected at identical MOI; 42h post infection, cells were stained with crystal violet and poliovirus plaque diameters were measured. The box indicates the data between the interquartile range (IQR) between the 25^th and 75^th percentile, the median is indicated by the thick line within the box. The thin vertical bars represent the 95th percentiles. (C, D) Quantification of poliovirus titers after a single round of infection at MOI of 0.1 (C) and MOI of 10 (D). Poliovirus titers from RACK1 KO #1 cells are decreased to HAP1 wildtype and RACK1-FLAG addback cells. The box indicates the data between the interquartile range (IQR) between the 25^th and 75^th percentile, the median is indicated by the thick line within the box. The thin vertical bars represent 95th percentiles. * p-value < 0.05; ** p-value < 0.01; *** p-value < 0.001 (E) Virion concentrations in the media of a one-step growth curve were determined by plaque assay from wildtype, RACK1 KO #1 and RACK1-FLAG addback cells.