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. 2021 Jan 12;11:744. doi: 10.1038/s41598-020-80354-6

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Phage ELISA of Affimer clones and LC–MS analysis of the purified CEA-Affimer protein. (A) Forty eight positive clones were incubated in wells coated with biotinylated CEA (blue bars) whilst biotinylated targets were omitted in the negative control wells (red bars); (B–D) the mass spectra of the purified CEA-Affimer I to III displaying the monomer and dimer peaks.