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. 2021 Jan 12;12:290. doi: 10.1038/s41467-020-20461-0

Fig. 2. Generation and characterization of Slc46a3−/− mice.

Fig. 2

a Scheme for the generation of Slc46a3−/− mice. b Southern blot genotyping of Slc46a3−/− mice using a specific probe. A representative image is shown from three wild-type (WT) and Slc46a3−/− mice. c Northern blot analysis of Slc46a3 mRNA in the livers of wild-type (WT) and Slc46a3−/− mice after treatment with TCDD (200 µg/kg) for 24 h. A representative image is shown from three  mice in each treatment group from both mouse lines. d Change in the color of the liver before and after perfusion in WT and Slc46a3−/− mice. e Representative H&E staining of liver tissue (n = 3 images per mouse). f Localization of eGFP-SLC46A3 in the lysosomes of Hepa1c1c7 cells. A representative florescence image was obtained from three biologically independent experiments. Blue color, nucleus; scale bar, 10 μm. g Purification of eGFP-SLC46A3-embedded lysosomes. IgG-L immunoglobulin light chain; IgG-H immunoglobulin heavy chain. The representative western blot image was obtained from three independent experiments. h Western blotting of eGFP-SLC46A3 using lysates of Hepa1c1c7 cells.