Fig. 7. SLC46A3 overexpression triggers TG accumulation in the liver.

a Oil Red O staining after adenovirus-based (upper, n = 5 per group) and hydrodynamic injection-based (lower, n = 3 per group) expression of Slc46a3 mRNA in the liver. b Hepatic Slc46a3 mRNA expression (left) and TG level (right) after administration of Ad-eGFP-Cre (n = 3 mice per group) or Ad-Slc46a3 (n = 5–6 mice per group). c Hepatic Adfp mRNA expression after Ad-Slc46a3 and control Ad-Cre administration  (n = 3 per each group). d Representative fluorescence images in the eGFP-adipophilin/mcherry-SLC46A3-expressing primary hepatocytes. The representative images were obtained from three biologically independent experiments. Scale bar, 10 μm; N nucleus (blue color). e Relative volume of lipid droplets in the eGFP (n = 11 counts per cell) or eGFP-SLC46A3 (n = 32 counts per cell) expressing primary hepatocytes from (d). f Nile Red staining after adenoviral induction of SCL46A3 in primary hepatocytes with Ad-Slc46a3. The representative fluorescence images were obtained from three biologically independent experiments. Scale bar, 10 μm. g Western blotting of phosphorylated AMPKα and ACC using the mice liver after viral induction of Ad-Cre-GFP or Ad-Cre-Slc46a3. The representative western blot images were obtained from three biologically independent experiments. Each data point represents the mean ± SD and the adjusted p value, presented in the panels, was determined by unpaired two-tailed Student’s t test using indicated sample sizes and groups.