Figure 7.

Chromosome A/B compartments are not substantially altered by T cell activation. (A) Pie charts showing the distribution of chromatin regions in the four different group of compartment changes: stable (A to A, B to B) or flipping (A to B and B to A). This was defined by pairwise comparison between activated and resting T cells. (B) PCA (principal component analysis) scores in resting and activated T cells along chromosome 1 representing the stability of the A/B compartments. PCA scores were calculated as described in “Methods”. (C) Boxplots showing the distribution of chromatin accessibility and gene expression at regions that exhibit A/B compartment shifts upon T cell activation. Shown are the median (central horizontal line), interquartile range (boxes), values of the upper and lower quartiles (whiskers), outliers beyond 1.5 IQR (circles). Statistics were determined with the unpaired Wilcoxon test.