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. 2021 Jan 12;11:826. doi: 10.1038/s41598-020-80637-y

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Corticosterone enhances alcohol-induced barrier dysfunction and mucosal inflammatory responses in the mouse colon. Adult mice were fed a liquid diet with (EF) or without (PF) ethanol for four weeks. In some groups, animals were injected with corticosterone (CORT) daily. Animals in other groups were injected with the vehicle. (A) Body weights were recorded twice a week. (B) Corticosterone levels were measured in plasma. (C) Mucosal permeability was measured by the vascular-to-luminal flux of FITC-inulin in the colon in vivo. (D,E) Epithelial junctional integrity was assessed by staining the cryosections of the colon for occludin and ZO-1 for tight junction (D) and E-cadherin and β-catenin for adherens junction (E) by immunofluorescence method followed by confocal imaging. (F–J) RNA isolated from colonic mucosa was analyzed for mRNA specific for TNFα (D), IL-1β (E), MCP1 (F), and IL-10 (G) by RT-qPCR. Values in graphs are mean ± SEM (n = 4–6 for A–C and 4 for F–J). Dots in bars indicate individual values. The numbers above the bars are p-values for differences between the groups indicated by the horizontal lines, and "ns" indicates no significant difference between groups.