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. 2021 Jan 12;11:826. doi: 10.1038/s41598-020-80637-y

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Corticosterone exacerbates alcohol-induced endotoxemia and systemic inflammation. Adult mice were fed a liquid diet with (EF) or without (PF) ethanol for four weeks. In some groups, animals were injected with corticosterone (CORT) daily. Animals in other groups were injected with the vehicle. (A) Endotoxemia was assessed by measuring plasma LPS levels. (B–D) Levels of TNFα (B), IL-1β (C), IL-6 (D), and MCP1 (E) were measured in plasma by ELISA. Values in graphs are mean ± SEM (n = 6, except 4 for the PF-Vehicle group). Dots in bars indicate individual values. The numbers above the bars are p-values for differences between the groups indicated by the horizontal lines, and "ns" indicates no significant difference between groups.