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. 2021 Jan 12;12:326. doi: 10.1038/s41467-020-20665-4

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a UCP1 expression in BAT and iWAT of DsbA-L^CD4-KO mice (n = 7) and control littermates (n = 5) fed an HFD for 12 weeks. b Immunohistochemical staining of UCP1 in BAT of DsbA-L^CD4-KO mice and control littermates fed an HFD for 12 weeks. Scale bars: 50 μm. c Thermogenic gene expression in BAT and iWAT of DsbA-L^CD4-KO mice (n = 7) and control littermates (n = 4) fed an HFD for 12 weeks. d The oxygen consumption of DsbA-L^CD4-KO mice (n = 8) and control littermates (n = 7) fed an HFD for 5 weeks was measured. e The average of oxygen consumption and energy expenditure between DsbA-L^CD4-KO mice and control littermates fed an ND (n = 4/group) or HFD (n = 7–8/group) for 5 weeks. f The basal OCR of BAT (n = 5/group) and iWAT (n = 4/group) collected from DsbA-L^CD4-KO mice and control littermates fed an HFD for 5 weeks. g mRNA levels of genes involved in mitochondrial and fatty acid metabolism in the BAT (n = 4–5/group) and iWAT (n = 3–4/group) of DsbA-L^CD4-KO mice and control littermates fed an HFD for 5 weeks. h Western blot analyses of UCP1 and PGC1α in the BAT collected from DsbA-L^CD4-KO mice (n = 6) and control littermates (n = 5) fed an HFD for 5 weeks. i mRNA levels of genes involved in thermogenesis in the BAT (n = 4–5/group) and iWAT (n = 4/group) of DsbA-L^CD4-KO mice and control littermates fed an HFD for 5 weeks. j The oxygen consumption of DsbA-L^CD4-KO mice (n = 5) and control littermates (n = 4), which were fed an HFD for 5 weeks and then tested under thermoneutral conditions (30 °C). k The average of oxygen consumption and energy expenditure between DsbA-L^CD4-KO mice (n = 5) and control littermates (n = 4) which were fed an HFD for 5 weeks and then tested under thermoneutral conditions (30 °C). Data shown are representative of three independent experiments. All data are presented as mean ± SEM. Statistical values p < 0.05 (*), p < 0.01 (**), p < 0.001 (***) are determined by two-tailed unpaired Student’s t-test (a (right panel), c, f, g, h (right panel), i or ANCOVA test (e, k)). Source data are provided as a Source Data File.