Fig. 5. DsbA-L deficiency in T cells suppresses IFN-γ production while promoting Treg cell accumulation.

DsbA-L^CD4-KO mice and control littermates were fed an HFD for 5 weeks. a Gating strategies of T cell subsets in adipose tissues. b Representative FACS plots of CD4⁺IFN-γ⁺ Th1 cells (left panel) and quantification of the frequencies and total numbers of CD4⁺IFN-γ⁺ Th1 cells (right panel). (n = 4/group). c Representative FACS plots of CD8⁺IFN-γ⁺ T cells (left panel) and quantification of the frequencies and total numbers of CD8⁺IFN-γ⁺ T cells (right panel). (n = 4/group). d, e Quantification of the frequencies and total numbers of Th2 cells (d) and Treg cells (e). (n = 4/group). f mRNA levels of T cell-associated cytokines in the SVF of BAT. (n = 5/group). g Protein levels of IFN-γ in the BAT and iWAT. (n = 4/group). h Protein levels of IFN-γ in the serum of DsbA-L^CD4-KO mice (n = 10) and control littermates (n = 12). Data shown are representative of three independent experiments. All data are presented as mean ± SEM. Statistical values p < 0.05 (*), p < 0.01 (**), p < 0.001 (***) are determined by two-tailed unpaired Student’s t-test. Source data are provided as a Source Data File.