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. 2021 Jan 12;11:746. doi: 10.1038/s41598-020-80608-3

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Histological evaluation of bone regeneration in rat calvarial defects at 4 and 8 weeks postoperatively: (A) Image for hematoxylin and eosin staining and Masson’s trichrome staining. Yellow background shows where defects were created. Semi-quantitative analysis of red-stained area ratio (%) of Masson’s trichrome staining in (B) 4 weeks and (C) 8 weeks using ImageJ software (V1.8.0_172, NIH, USA) (https://imagej.nih.gov/ij/). CB, cortical bone; LB, lamellar bone; WB, woven bone; F, fibrous tissue; IC, inflammation cell; S, scaffold; BM, bone marrow; DI, diploe; V: vessels. (×40 and ×100 magnification, scale bar = 200 μm. *p < 0.05; ***p < 0.001 versus control group; Two-way krANOVA test).