Skip to main content
. 2021 Jan 12;11:621. doi: 10.1038/s41598-020-77808-2

Figure 3.

Figure 3

Effect of REMFS on levels of sAPPα and sAPP in human brain cultures at DIV7 and DIV28. Cultures were grown for 7 or 28 days and then exposed as described in the text for 14 (DIV7) or 4 or 8 days (DIV28), as described in the text. CM was assayed for levels of sAPP or sAPPα. sAPPα was measured by ELISA. Total sAPP was measured by semi-quantitative western blotting. (A) Schedule of cell growth and treatments for data presented in figure. (B) sAPPα was measured by ELISA of CM from PHB-DIV7 exposed to REMF for 14 day as described in the text. REMFS does not cause a significant change in levels of sAPPα. (C) Total sAPP was measured by semiquantitative western blotting of CM from PHB-DIV28 exposed to REMFS at 64 MHz with SAR of 0.9 W/kg for 4 or 8 days, as described in the text. (D) Analysis of blot densitometry revealed that, while the interval between 28 + 4 and 28 + 8 days significantly increased sAPP, there was no effect of REMFS treatment.