Figure 4.

Sustained OHT results in ocular stretch. (A) OHT eyes demonstrated a significant increase in diameter by 14 days compared to both pre-surgery size and NT controls (n = 31 NT eyes; n = 35 OHT eyes, of which 20 were measured preoperatively, pre-OHT). (B, C) Eye diameter at the limbus and axial length at day 14 demonstrated reasonable correlation to measures of IOP (AUC, mean, peak), indicating a relationship between the magnitude of IOP increase and increase in the size of the eye. (D) These correlations were not driven by animal age or weight. (E) To assess retinal stretch following OHT, whole eyes were paraffin embedded and sectioned, and RGCs were labeled with antibodies against RBPMS (n = 6 NT eyes; n = 8 OHT eyes). There was no significant increase in total retinal length following OHT, which was highly variable even across NT eyes. RGC counts across the retina confirmed gross RGC loss. Scale bars: 1 mm (upper panel), 20 µm (lower panel). (F) RGC density was highly correlated with all measured of IOP and eye size. (G) Retinal length correlated with measures of IOP but to a lesser degree. For complete n values, please see the Methods section. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001; NS, non-significant (P > 0.05).