Figure 2.

(A) NG2 immunostaining was analyzed using unpaired Student's t‐test with Welch's correction to measure the number of oligodendrocyte progenitor cells in the ischemic border zone. Treatment of T1DM stroke with 3 µg/kg VT significantly increases the number of NG2 positive oligodendrocyte progenitor cells compared to PBS‐treated T1DM‐MCAo rats. (B‐D) Immunostaining with Myelin basic protein (MBP) and Sternberger monoclonal clone 32 (SMI‐32) was used to evaluate the integrity of myelin and degree of demyelination, respective and analyzed using unpaired Student's t‐test with Welch's correction. Treatment of T1DM stroke with 3 µg/kg VT significantly increases MBP expression and decreases SMI‐32 expression in the striatal white matter in ischemic boundary zone compared to PBS‐treated T1DM‐MCAo rats. The ratio of the SMI‐32/MBP was significantly decreased in T1DM stroke rats receiving 3 µg/kg VT treatment compared to PBS treatment, indicating that VT treatment improves white matter integrity in T1DM stroke rats. Arrows indicate positive staining. N = 6–8 animals per group. **p < 0.01, ***p < 0.001