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. 2021 Jan 12;12:295. doi: 10.1038/s41467-020-20527-z

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig 6 — a Fold change of indicated mRNAs in A549 cells with or without RIG-I knockdown. n = 3 biologically independent samples. b Analysis for circNDUFB2 enrichment. RIP assay was performed using RIG-I antibody in A549 cells. POLR2A was used as a positive control and circSMARCA5 was used as a negative control. n = 4 biologically independent samples. c Protein levels in A549 cells with circNDUFB2 overexpression or RIG-I knockdown. d Protein levels of IRF3 and P65 in the nuclear and cytoplasmic fractions in the indicated cells. e Levels of CXCL10, CXCL11, CCL5, and IFNβ in the supernatants of A549 cells with circNDUFB2 overexpression or RIG-I knockdown. n = 3 biologically independent samples. f Interactions between CARDs of RIG-I with helicase domain of RIG-I and MAVS in A549 cells. FLAG-CARD, CARDs of RIG-I with FLAG-tagged; HA-ΔCARD, helicase and CTD of RIG-I with HA-tagged. g The volume and weight of subcutaneous xenograft tumors (n = 5 mice per group). h Levels of IFNβ in the serum of mice (n = 5 mice per group). i The percentage of CD8⁺ T cells and DCs in subcutaneous xenograft tumors. n = 5 tumors per group. j Correlation analysis for circNDUFB2 and RIG-I or IFNβ in the tumorous tissues of 52 NSCLC patients. ΔCt values were normalized according to GAPDH. n = 52 biologically independent NSCLC tissues. R represents the Pearson correlation coefficient. Data are presented as mean ± s.d. P values are calculated by unpaired two-sided t-test in a, b, e, g–i. P values in j are calculated by F-test. Two independent experiments were carried out with similar results in c, d, f.