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. 2021 Jan 12;11:472. doi: 10.1038/s41598-020-78778-1

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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(a) Pre-ablation SEM image of the coral sector analysed by LA; (b) δ¹¹B map of coral area, where yellow colours show high δ¹¹B values (> 25‰), green colours show intermediary δ¹¹B values (c.21–25‰) and blue colours show low δ¹¹B values (< 21‰); (c) Overlay of δ¹¹B map and SEM image, the values clearly match the structural elements of the coral with high values being confined to the polyp walls and low values to the columella. Sub-annual variability of ~ 1‰ is visible in the wall edges which may correspond to the dissepiments (not-visible). The two walls analysed show very similar trends but at different absolute values implying a potential 3-dimensional component to the analyses. (d) Post-ablation SEM image of the coral area ablated.