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. 2021 Jan 12;9:12. doi: 10.1186/s40168-020-00967-x

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 7 — Location-specific alterations of gut microbial proteolytic motif in the MLI metaproteome of IBD. a and d ascending colon, b and e descending colon, and c and f terminal ileum. Hierarchical clustering analysis (a–c) and partial least squares discriminant analysis (PLS-DA, d–f) of altered amino acid frequencies around the cleavage site in IBD. Group averages are shown in the heatmap. Dunn-Bonferroni post hoc analysis following Kruskal-Wallis test was employed to detect significant difference among three groups (CD, Ctrl, and UC). *P < 0.05 versus Ctrl; **P < 0.01 versus Ctrl; ***P < 0.001 versus Ctrl; ^#P < 0.05 (CD versus UC); ^##P < 0.01 (CD versus UC); ^###P < 0.001 (CD versus UC)