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. 2021 Jan 13;12:55. doi: 10.1186/s13287-020-02109-4

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 7 — a Representative confocal microscope images of hiPSC aggregates taken from the final day of the vertical-wheel bioreactor serial passage (day 12) and stained for pluripotency markers SSEA-4, TRA-1-60, and Nanog (scale bar = 100 μm) and b differentiated into neuronal cells (immuno-stained for β-tubulin and PAX6), hepatocytes (immuno-stained for HNF4α), and cardiomyocytes (immuno-stained for TNNT2) (scale bar = 50 μm)