Table 1.
Molecular and IHC data for NOTCH2-mutated DLBCL cases
| DLBCL case ID | Notch2 sequence variant29 | Variant reads/total (%) | LymphGen subtype34 | Cell of origin32 | BCL6 translocation | BCL6 IHC* | NICD2 IHC, % POS cells* | Total Notch2 IHC, % POS cells* |
|---|---|---|---|---|---|---|---|---|
| DLC_0048 | p.H2293Efs*21 | 38/289 (13) | EZB/A53 | UNCL | NEG | POS (strong) | 0 | 0 |
| DLC_0060 | p.R2400* | 9/25 (36) | Other | GCB | POS | POS (strong) | 0 | 0 |
| DLC_0201 | p.Q2367* | 69/173 (40) | BN2 | ABC | POS | POS (weak, subset) | 0 | 0 |
| DLC_0231 | p.Q2361* | 6/21 (29) | BN2/A53 | ABC | POS | POS (strong) | 0 | 10 |
| DLC_0239 | p.I2304Hfs*9 | 292/400 (73) | Other | UNCL | NEG | NEG (weak, subset) | 0 | 30 |
| DLC_0242 | p.T2355Hfs*45 | 2/4 (50) | ST2 | GCB | NEG | POS (strong) | 0 | 0 |
| DLC_0269 | p.Y2383* | 93/194 (48) | BN2 | GCB | POS | POS (strong) | 0 | 30 |
| DLC_0319 | p.E2290Kfs*5 | 10/26 (38) | A53 | ABC | NEG | POS (weak, subset) | 0 | 0 |
| DLC_0330 | p.R2400* | 9/46 (20) | MCD | ABC | POS | POS (strong) | 0 | 0 |
ABC, activated B cell; GCB, germinal center B; ID, identifier; NEG, negative; POS, positive; UNCL, unclassifiable.
*
See supplemental Figure 4 for representative staining results.