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[Preprint]. 2021 Jan 9:2021.01.09.426034. [Version 1] doi: 10.1101/2021.01.09.426034

Figure 2. Comparison of poly I:C preparations.

Figure 2.

(a) Plasma concentration of the pro-inflammatory cytokines IL-1β, IL-6, TNFα, IFNβ were determined using ELISA. (b): Transcription of the pro-inflammatory cytokines IL-1β, IL-6, TNFα, IFNβ in hippocampal (HPC) tissue were determined using real time PCR. Experimental groups consisted of in C57BL/6J female adult mice 3h post-treatment with saline (n=3), 20mg/kg Amersham poly I:C (n=5), 20mg/kg Sigma poly I:C (n=5), 20mg/kg HMW Invivogen poly I:C (n=5) and LMW Invivogen poly I:C (n=5). (c) 0.5% agarose gel showing approximate length of different preparations in base pairs (bp). S1, S2 represent 2 different batches of Sigma polyI:C; Am represents Amersham poly I:C; LMW: In vivogen low molecular weight, HMW: Invivogen high molecular weight. All data are represented as mean +/− SEM and were compared using one-way ANOVA, followed by Bonferroni post-hoc tests. For plasma cytokines, Bonferroni tests compared differences between Sigma poly I:C versus all other poly I:C groups, and differences are denoted by *p<0.05, **p<0.01 and ***p<0.001. For hippocampal transcripts increases were smaller and more variable so Bonferroni tests were performed to compare different poly I:C treatments to saline-treated animals and differences are denoted by *p<0.05.