Figure 4. Time course analysis of innate responses to poly I:C of different MWs.

a) Systemic secretion of the cytokines IL-6, TNFα and IFNβ was determined using ELISA in female adult mice following treatment with saline (n=4 per time-point), 20mg/kg (n=6 per time-point) and 80mg/kg Sigma poly I:C (n=5–6 per time-point). b) The same animals were used for isolation of hypothalamic RNA for cDNA synthesis and qPCR for expression of key pro-inflammatory transcripts and type I interferon pathway transcripts. c) The same animals were used for isolation of hippocampal RNA to verify these patterns in hippocampus. All data are represented as mean ±SEM and were compared using two-way ANOVA, followed by Bonferroni post-hoc tests comparing differences between saline and both poly I:C groups (⁺ p<0.05, ⁺⁺ p<0.01 and ⁺⁺⁺ p<0.001) and between the 2 different poly I:C preparations (*p<0.05, **p<0.01, ***p<0.001, ****p<0.0001).