Table 2. Crystallization.

Method	Hanging drop
Plate type	24-well plate
Temperature (K)	277
Protein concentration (mg ml−1)	2.0
Buffer composition of protein solution	20 mM Tris–HCl pH 8.0, 200 mM NaCl, 1 mM TCEP
Composition of reservoir solution	0.2 M potassium bromide, 0.2 M potassium thiocyanate, 0.1 M Tris–HCl pH 7.8, 2.6% γ-PGA (Na+ form, LM), 2.4%(w/v) PEG 20 000
Volume and ratio of drop	3 µl, 1:1 ratio of protein:reservoir solution
Volume of reservoir (ml)	1