Table 2. Crystallization.

Method	Hanging drop
Plate type	EasyXtal 15-well (Qiagen)
Temperature (K)	293
Protein concentration (mg ml−1)	10–12
Buffer composition of protein solution	25 mM Tris pH 8, 200 mM NaCl, 2 mM DTT, 0.5 mM EDTA, 5% glycerol
Composition of reservoir solution	0.1 M Bicine pH 9, 7–10%(v/v) MPD, 3–5%(v/v) tert-butanol
Volume and ratio of drop	2 µl, 1:1
Volume of reservoir (µl)	500