Table 1. Macromolecule-production information.

Source organism	Homo sapiens
DNA source	Mammalian Gene Collection clone ID 3876632
Forward primer†	5′-CGCGGATCCATGTGCGACAAGGAGTTCATG-3′
Reverse primer‡	5′-CCGGAATTCTCACTGGAGAAGAGCTTTGATTGC-3′
Cloning vector	pGEX-6P-1
Expression vector	pGEX-6P-1
Expression host	E. coli BL21 (DE3)
Complete amino-acid sequence of the construct produced§	MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLEVLFQ GPLGS MCDKEFMWALKNGDLDEVK DYVAKGEDVNRTLEGGRKPLHYAADCGQLEILEFLLLKGADINAPDKHHITPLLSAVYEGHVSCVKLLLSKGADKTVKGPDGLTAFEATDNQAIKALLQ

^†The BamHI site is underlined.

^‡The EcoRI site is underlined.

^§The GST-tag sequence is in italics, the human V-1 sequence is underlined and the HRV 3C protease cleavage site is in bold.