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. 2021 Jan 5;7(1):183–199. doi: 10.1021/acscentsci.0c01405

Figure 1.

Figure 1

Construct design for SARS-CoV-2 spike-functionalized ferritin nanoparticles. All constructs are based on the Wuhan-Hu-1 amino acid sequence (GenBank MN9089473) of SARS-CoV-2 spike. Spike-functionalized ferritin constructs were made by fusing spike ectodomain (residues 1–1213) or spikeΔC (residues 1–1143) to the H. pylori ferritin subunit separated by an SGG linker. A structural representation based on the spike trimer cryo-EM structure (PDB 6VXX) and the H. pylori ferritin crystal structure (PDB 3BVE) depicts the 24-subunit particle displaying spike or spikeΔC on the surface. The estimated size of the spike-functionalized ferritin particles based on structural data is ∼300 Å. The S-GCN4 and SΔC-GCN4 trimer constructs were made by fusing either the full-length spike residues (1–1213) or spikeΔC (1–1137) to a modified GCN4 trimerization domain followed by a hexahistidine tag. A structural representation of the spike trimers based on the cryo-EM structure (PDB 6VXX) is shown with an estimate length of ∼100 Å. The RBD spans residues 319–541 of the spike protein and is preceded by the native signal peptide (not shown) and followed by a hexahistidine tag.