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. 2020 Nov 9;143(12):3699–3716. doi: 10.1093/brain/awaa320

Figure 3.

Figure 3

Upregulation of O-GlcNAcylation in dopamine neurons does not negatively affect neuronal structures. (A) Schematic illustration depicting selective upregulation of O-GlcNAc in dopamine neurons. (B) Body size and weight in DAT-Cre;Oga+/+ and DAT-Cre;Ogafl/fl mice. (C) Midbrain TH+ dopaminergic neurons in adult DAT-Cre;Oga+/+ and DAT-Cre;Ogafl/fl mice. (D) Confocal images of TH and O-GlcNAc immunofluorescence in SNc. (E) Summary statistics of TH and O-GlcNAc immunoreactivities. (F and G) Representative confocal images of a single dopamine neuron in SNc and VTA by intracellular biocytin labelling. (H) Sholl analysis for the number of dendrite intersections in SNc and VTA dopamine neurons. (I) Total number of intersections in SNc and VTA dopamine neurons. (J) Representative confocal images of dendrite and dendritic spines in SNc and VTA dopamine neurons. (K) Quantification of spine density from dopamine neurons. (L) Low magnification images of striatum showing TH+ dopamine axons in adult DAT-Cre;Oga+/+ and DAT-Cre;Ogafl/fl mice. (M) Confocal images of TH, DAT, and O-GlcNAc immunofluorescence in DLS. (N) Summary statistics of O-GlcNAc, TH, and DAT immunoreactivities in axon fibres of DLS. Data were analysed by ordinary two-way ANOVA (B), unpaired t-test (E, I, K and N) and repeated measures two-way ANOVA (H). Data are represented as mean ± SEM. *P < 0.05, ****P < 0.0001.