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. 2020 Nov 6;143(12):3827–3841. doi: 10.1093/brain/awaa317

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© The Author(s) (2020). Published by Oxford University Press on behalf of the Guarantors of Brain.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Allelic discrimination plots of the digital droplet PCR for the R42H TARDBP somatic variant. Each marker represents a single droplet and its respective wild-type (horizontal axis) and variant (vertical axis) signal intensity. Five different tissues of the carrier were tested: blood, middle temporal gyrus (MTG), middle frontal gyrus (MFG), lateral parietal lobe (LPS), cerebellum (CER) and a negative control of water is shown. The grey droplets are considered empty, green droplets are wild-type only, orange is both wild-type and variant alleles, and blue droplets were harbouring only the variant allele.