. 2021 Jan 13;16(1):e0245160. doi: 10.1371/journal.pone.0245160

Table 1. The details of the primers used for T-ARMS-PCR, RSM, and HRM analysis.

	Mutations	Sequence (5′->3′) forward and reverse	Ta	Product (bp)
T-ARMS-PCR	A395T	FOP: `TAGCATGGCAATTACTTTACTCCTTA`	60°C	Outer primers: 474
		ROP: `GTTGAGTTTTCATAACACTAGCAACAC`		A allele: 212
		A allele: `ACTCCTGTTTTTGGTAAAGGTGTTATATA`		A allele: 212
		A allele: `ACTCCTGTTTTTGGTAAAGGTGTTATATA`		T allele: 318
		T allele: `CCATTAATCTAGAGTTTGGACAATGAA`		T allele: 318
	C461T	FOP: `AAAGATAGAGTTCCAATGGTTTTCTACTGG`	60°C	Outer primers: 536
		ROP: `TCAGCATACAATTGAGCAAATGATCT`		C allele: 237
		C allele: `TTTGCTAAATTTGCTTTGACTACTGAGTC`		T allele: 355
		T allele: `TGATCTTTGGAACATAGGTTTTGACAA`
RSM	C461T	FP: `TCTACTGGATCCCATGGTTTGG`	60°C	Amplicon: 571
RSM	C461T	RP: `TGAGGTAATGGCAAGTTTGGG`
HRM	A395T & C461T	FP: `ACTCCTGTTTTTGGTAAAGGTGT`	60°C	Amplicon: 131
HRM	A395T & C461T	RP: `ACTTCTTCTCTGATCTTTGGAACA`	60°C	Amplicon: 131

FOP: Forward outer primer; ROP: Reverse outer primer, FP: Forward outer primer; RP: Reverse primer; Ta: Annealing Temperature, bp: Base pairs