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. 2021 Jan 13;16(1):e0245160. doi: 10.1371/journal.pone.0245160

Table 3. Comparison between DNA sequencing, T-ARMS-PCR, RSM, and HRM approaches.

DNA sequencing T-ARMS-PCR RSM* HRM
Sensitivity 100% 100% 100% 100%
Specificity 100% 100% 100% 94.74%
Detection time ~24 hours ~4 hours ~5 hours ~3 hours
Cost/reaction ~15 US dollars <1 US dollars ~2 US dollars <1 US dollars
Detection limit 5 ng 10 ng 1 ng 0.1 ng

* Only for C461T mutation