Table 3. Comparison between DNA sequencing, T-ARMS-PCR, RSM, and HRM approaches.
DNA sequencing | T-ARMS-PCR | RSM* | HRM | |
---|---|---|---|---|
Sensitivity | 100% | 100% | 100% | 100% |
Specificity | 100% | 100% | 100% | 94.74% |
Detection time | ~24 hours | ~4 hours | ~5 hours | ~3 hours |
Cost/reaction | ~15 US dollars | <1 US dollars | ~2 US dollars | <1 US dollars |
Detection limit | 5 ng | 10 ng | 1 ng | 0.1 ng |
* Only for C461T mutation