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. Author manuscript; available in PMC: 2021 Aug 1.
Published in final edited form as: Biotechnol Bioeng. 2020 Jun 2;117(8):2540–2555. doi: 10.1002/bit.27383

Figure 1. Biocompatibility of epithelial and mucus gel layers formed on a transwell membrane.

Figure 1.

(A) Macroscopic, bottom-view images of the translucent membrane without mucus (top left) and the opaque mucus layer after gel formation (bottom left). Tiled microscopic image of the transwell membrane with RFP-expressing HT-29 cells covering the entire membrane without (top right) and with a mucus layer (bottom right). (B) Side view of transwell inserts demonstrating sol (left, top) to gel (left, bottom) transition of PSIM at 0 and 12 hours. Microscopic image of a 20 μm thick histological cross-section of the transwell membrane covered with HT-29 cell layer (middle) and PSIM gel layer formed on top (right). (C) A mucus gel layer formed 12 hours after addition of solubilized PSIM (20 mg/ml) and incubation at 37 °C. (D) Live (Green) and Dead (Red) cell staining of the epithelial cell layer on a transwell membrane. (E) Quantified area covered by GFP positive and RFP positive signals showed no significant difference with or without mucus (n = 3). (F) Confocal microscope image of a DAPI-stained PSIM gel layer (red) with embedded GFP-expressing Salmonella (green).