Skip to main content
. Author manuscript; available in PMC: 2021 Aug 1.
Published in final edited form as: Biotechnol Bioeng. 2020 Jun 2;117(8):2540–2555. doi: 10.1002/bit.27383

Figure 2. Immunological response of epithelial and immune cells to PSIM.

Figure 2.

(A) Schematic depiction of the transwell insert placed in 24 multi well plates. HT-29 cells (blue) were cultured on the apical side of the transwell membrane and cell culture medium was added to the apical (100 μl) and basolateral (600 μl) side of the cells (w/o mucus). Reconstituted mucus at 20 mg/ml concentration (brown, 100 μl) layered on top of the epithelial cells (w mucus). (B) THP-1 cells cultured in 24 multi well plates and cell culture media (w/o mucus, 100 μl) or reconstituted mucus (w/ mucus, 100 μl) added directly to the culture medium (600 μl). (C) Mucus increased chemokine (IL-8) secretion from HT-29 cells (*, P < 0.05; n = 3) and (D) no change in cytokine (TNF-α) secretion. Mucus did not cause a significant change in (E) IL-8 or (F) TNF-α concentration in THP-1 culture medium (n = 3).