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. Author manuscript; available in PMC: 2022 Jan 6.
Published in final edited form as: Neuron. 2020 Nov 11;109(1):73–90.e7. doi: 10.1016/j.neuron.2020.10.027

Figure 6. Targeted CCK neurons convey mechanical allodynia induced by both inflammatory and neuropathic pain models.

Figure 6.

(A-B) Schematic of CCKCre mice injected with AAV8.hSyn.FLEX.PSAM-GlyR virus. Dorsal horn shows α-BTX-Alexa647 binding (red) in lamina III with scattered cells in laminae II, IV and V. No overlap with PKCγ (green) or CR (blue) in laminae II or III. Yellow box shows location of insert. Arrow shows example of non-colocalized cell. Scale bars = 100 μm and 20 μm.

(C) PSEM89S injection in CFA model partially reverses punctate (p=0.0051) and dynamic (p=0.0185) allodynia. N= 6 mice.

(D) PSEM89S injection at 2 and 6 weeks after sural-SNI partially reverses punctate (p=0.0264, 2wk; p=0.0003, 6wk) and completely reverses dynamic (p=0.0155, 2wk; p=0.0022, 6wk) allodynia. N=7 mice.

(E) Summary of the role of targeted CCK neurons in conveying mechanical allodynia in persistent pain models.

See also Figure S4.