KEY RESOURCES TABLE.
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Guinea Pig polyclonal anti-PKCγ (1:500, IHC) | Frontier Institute | AF350 |
| Mouse monoclonal anti-Calretinin (1:1000, IHC) | Swant | RRID: AB_10000320 |
| Mouse monoclonal anti-NeuN (1:1000, IHC) | Chemicon | RRID: AB_2298772 |
| anti-hPax2 Affinity Purified Goat IgG (1:200, IHC) | R&D Systems | RRID: AB_10889828 |
| Chicken anti-GFP (1:1000, IHC) | Life Technologies | RRID: AB_2534023 |
| Rabbit anti-GFP (1:1000, IHC) | Molecular Probes | RRID: AB_221570 |
| α-Bungarotoxin Alexa Fluor 647 conjugate (1:3000, IHC) | Life Technologies | Cat: B35450 LOT: 1712139 |
| α-Bungarotoxin Alexa Fluor 488 conjugate (1:3000, IHC) | Life Technologies | Cat: B13422 LOT: 1843685 |
| AffiniPure Donkey anti-Mouse DyLight 405 (1:500, IHC) | Jackson ImmunoResearch | RRID: AB_2340839 |
| AffiniPure Donkey anti-Mouse Alexa Fluor 488 (1:500, IHC) | Jackson ImmunoResearch | RRID: AB_2340846 |
| AffiniPure Donkey anti-Mouse Alexa Fluor 594 (1:500, IHC) | Jackson ImmunoResearch | RRID: AB_2340854 |
| AffiniPure Donkey anti-Mouse Alexa Fluor 647 (1:500, IHC) | Jackson ImmunoResearch | RRID: AB_2340862 |
| AffiniPure Donkey anti-Rabbit Alexa Fluor 488 (1:500, IHC) | Jackson ImmunoResearch | RRID: AB_2313584 |
| AffiniPure Donkey anti-Rabbit Alexa Fluor 594 (1:500, IHC) | Jackson ImmunoResearch | RRID: AB_2340623 |
| AffiniPure Donkey anti-Rabbit Alexa Fluor 647 (1:500, IHC) | Jackson ImmunoResearch | RRID: AB_2492288 |
| AffiniPure Donkey anti-Chicken Alexa Fluor 488 (1:500, IHC) | Jackson ImmunoResearch | RRID: AB_2340375 |
| AffiniPure Donkey anti-Goat DyLight 405 (1:500, IHC) | Jackson ImmunoResearch | RRID: AB_2340426 |
| AffiniPure Donkey anti-Goat Alexa Fluor 488 (1:500, IHC) | Jackson ImmunoResearch | RRID: AB_2340428 |
| AffiniPure Donkey anti-Goat Alexa Fluor 647 (1:500, IHC) | Jackson ImmunoResearch | RRID: AB_2340436 |
| AffiniPure Donkey anti-Guinea Pig DyLight 405 (1:500, IHC) | Jackson ImmunoResearch | RRID: AB_2340470 |
| AffiniPure Donkey anti-Guinea Pig Alexa Fluor 488 (1:500, IHC) | Jackson ImmunoResearch | RRID: AB_2340472 |
| AffiniPure Donkey anti-Guinea Pig Cy3 conjugate (1:500, IHC) | Jackson ImmunoResearch | RRID: AB_2340460 |
| AffiniPure Donkey anti-Guinea Pig Alexa Fluor 594 (1:500, IHC) | Jackson ImmunoResearch | RRID: AB_2340474 |
| AffiniPure Donkey anti-Guinea Pig Alexa Fluor 647 | Jackson ImmunoResearch | RRID: AB_2340476 |
| Bacterial and Virus Strains | ||
| AAV8.hSyn.DIO.EGFP | UNC Vector Core and Vigene Biosciences | N/A |
| AAV8.hSyn.FLEX.PSAML141F-GlyR.IRES.EGFP | UNC Custom | N/A |
| AAV8.hSyn.DIO.hM3d(Gq)-mCherry | UNC Roth Collection | N/A |
| AAVDJ.pCAG.FLEX.PSAML141F-GlyR | Vigene Biosciences Custom | N/A |
| Biological Samples | ||
| Chemicals, Peptides, and Recombinant Proteins | ||
| Clozapine-N-Oxide | Enzo Life Science | BML-NS105-0025 |
| PSEM89S (S)-2,5-Dimethoxy-N-(quinuclidine-3-yl)benzamide | Apex Scientific | 1221570-55-3 |
| λ-Carrageenan | Sigma Aldrich | 22049-5G-F |
| Capsaicin | Sigma Aldrich | M2028 |
| Complete Freund’s Adjuvant | Sigma Aldrich | F5881 |
| Allyl Isothiocyanate (Mustard Oil) | Sigma Aldrich | 377430 |
| Ketathesia | Henry Schein Animal Health | 056334 |
| AnaSed (Xylazine) | Henry Schein Animal Health | 033197 |
| Isothesia (Isoflurane) | Henry Schein Animal Health | 029405 |
| Formalin | Sigma | 181090010 |
| TSA Biotin System | Perkin Elmer | NEL700A001KT |
| Tamoxifen | Sigma-Aldrich | T5648 |
| Tat-γV5-3 | Dr. Mochley-Rosen. (Sweitzer et al 2004) | N/A |
| Critical Commercial Assays | ||
| Deposited Data | ||
| Experimental Models: Cell Lines | ||
| Experimental Models: Organisms/Strains | ||
| Ccktm1.1(cre)Zjh/J (CCKCre) | Jackson Laboratories | RRID: IMSR_JAX:012706 |
| Calb2tm1(cre)Zjh/J (CRCre) | Jackson Laboratories | RRID: IMSR_JAX:010774 |
| Tg(Slc17a8-icre)1Edw/SealJ (VGLUT3iCre) | Jackson Labs Rebecca Seal | RRID: IMSR_JAX:018147 |
| Tg(Slc17a8-EGFP) 1Edw/SealJ (VGLUT3eGFP) | Jackson Labs Rebecca Seal | RRID: IMSR_JAX:018148 |
| PKCymGFP | David Ginty (Abraira et al., 2017) | N/A |
| PKCγCreERT2 | David Ginty (Abraira et al., 2017) | N/A |
| B6.Cg-Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/J (tdTomato) | Jackson Laboratories | RRID: IMSR_JAX:007914 |
| Tlx3tm1(cre)Qima | Qiufu Ma (Xu et al., 2008) | N/A |
| C57BI/6J | Jackson Laboratories | RRID: IMSR_JAX:000664 |
| Oligonucleotides | ||
| qPCR primers, see Table S1 | This paper | N/A |
| Cholecystokinin (Cck) RNAscope probe (C2, C3 channels) | ACDBio | 402271 |
| Copine-4 (Cpne4) RNAscope probe (C1 channel) | ACDBio | 474721 |
| v-maf musculoaponeurotic fibrosarcoma oncogene homolog (avian) (Maf) RNAscope probe (C3 channel) | ACDBio | 412951 |
| Thyrotropin Releasing Hormone (Trh) RNAscope probe (C3 channel) | ACDBio | 436811 |
| Tachykinin precursor 1 (Tac1) RNAscope probe (C1 channel) | ACDBio | 410351 |
| Tachykinin2 (Tac2) RNAscope probe (C3 channel) | ACDBio | 446391 |
| Lamp5 RNAscope probe (C3 channel) | ACDBio | 451071 |
| Gastrin releasing peptide (Grp) RNAscope probe (C1 channel) | ACDBio | 317861 |
| Recombinant DNA | ||
| pCAG-Flex-TCB | Miyamichi et al., 2013 | Addgene (#48332) |
| Software and Algorithms | ||
| PRISM v7 and v8 | GraphPad | RRID: SCR_002798 https://www.graphpad.com/scientific-software/prism/ |
| NIS-Elements | Nikon | RRID: SCR_014329 https://www.microscope.healthcare.nikon.com/ |
| Fiji (ImageJ) | NIH | RRID: SCR_002285 https://imagej.nih.gov/ij/ |
| Axon pCLAMP v10 | Molecular Devices | RRID: SCR_011323 https://www.moleculardevices.com/products/axon-patch-clamp-system |
| Canvas X | Canvas GTX | RRID: SCR_014312 https://www.canvasgfx.com/en/products/canvas-x/ |
| Corel Draw X7 | Corel Corporation | RRID: SCR_014235 https://www.coreldraw.com/en/ |