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. Author manuscript; available in PMC: 2021 Jun 1.
Published in final edited form as: Curr Protoc Mol Biol. 2020 Jun;131(1):e122. doi: 10.1002/cpmb.122

Figure 10. Automatic mathematical calculation of the simultaneous equations.

Figure 10.

(A) Screenshot of the second worksheet of the provided Microsoft Excel template (see Supplemental File), including simplified instructions to facilitate the generation of the simultaneous equations needed to spectrally unmix the obtained measurements into distinct values for the D-Luciferin luciferases. Distinct values for the coelenterazine luciferases are similarly obtained (not shown). (B) Real example of the automatic calculation of the simultaneous equations needed to spectrally unmix the luminescence for the D-Luciferin luciferases into distinct values. The transmission coefficients obtained for Fluc as previously illustrated (see Figure 9) are highlighted. Distinct values for the coelenterazine luciferases are similarly obtained.