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. Author manuscript; available in PMC: 2021 Jun 1.
Published in final edited form as: Curr Protoc Mol Biol. 2020 Jun;131(1):e122. doi: 10.1002/cpmb.122

Figure 13. Development of multiplex luciferase assays for the collateral analysis of cellular signaling events.

Figure 13.

(A) Simplified schematic of the dual luciferase assay widely used for monitoring the activity of a single experimental cellular signaling event. (B) Simplified schematic of a multiplex hextuple luciferase assay that can be used for the collateral monitoring of five experimental cellular signaling events.