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. Author manuscript; available in PMC: 2021 Jun 1.
Published in final edited form as: Curr Protoc Mol Biol. 2020 Jun;131(1):e122. doi: 10.1002/cpmb.122

Figure 3. Quality control of luciferase-encoding plasmid DNA preparations.

Figure 3.

Purified plasmid DNA is analyzed by agarose gel electrophoresis two-ways: plasmid fingerprinting after restriction enzyme cutting (to confirm appropriate DNA banding patterns), and uncut (to confirm the absence of unwanted multimerizations). Plasmids are one of the six constitutive luciferases transcriptional units: ELuc (Plasmid 1), FLuc (Plasmid 2), RedF (Plasmid 3), NLuc (Plasmid 4), Renilla (Plasmid 5), and GrRenilla (Plasmid 6), and the multiplex luciferase reporter vector (Plasmid 7).