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. Author manuscript; available in PMC: 2021 Jun 1.
Published in final edited form as: Curr Protoc Mol Biol. 2020 Jun;131(1):e122. doi: 10.1002/cpmb.122
1x Each constitutive
luciferase vector (3x)
Multiplex luciferase reporter
vector (66x)
Vector (150 ng/μL) 1 μL 3 μL 66 μL
Opti-MEM 15 μL 45 μL 990 μL
P3000 reagent 0.3 μL 0.9 μL 19.8 μL
Lipofectamine reagent 0.3 μL 0.9 μL 19.8 μL