Simultaneous examination of cellular pathways using multiplex hextuple luciferase assaying

. Author manuscript; available in PMC: 2021 Jun 1.

Published in final edited form as: Curr Protoc Mol Biol. 2020 Jun;131(1):e122. doi: 10.1002/cpmb.122

	CONTROL + DMSO + 10mM Citric Acid, pH 3.0			+TGF-β 5 ng/mL			+TGF-β 5 ng/mL + 6 μM RepSox
	VALUE	ERROR	N	VALUE	ERROR	N	VALUE	ERROR	N
Log₂(TGF-β)	0	0.1765	4	0.8669	0.0952	4	0.0800	0.0676	4
Log₂ (NF-κβ)	0	0.0636	4	0.6045	0.0668	4	1.0025	0.0081	4
Log₂(P53)	0	0.0690	4	-0.6201	0.0937	4	0.2686	0.0700	4
Log₂(c-MYC)	0	0.0948	4	-0.1153	0.0369	4	0.4957	0.0685	4
Log₂(MAPK/JNK)	0	0.0592	4	0.0820	0.0193	4	-0.0542	0.0392	4