Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Aedes aegypti) | fruitless | RefSeq | LOC5567734 | |
| Gene (Anopheles gambiae) | fruitless | VectorBase | AGAP000080 | |
| Gene (Culex quinquefasciatus) | fruitless | RefSeq | CPIJ010853 | |
| Gene (Wyeomyia smithii) | fruitless | This paper | Figure 1—source data 1 | |
| Gene (Toxorhynchites amboinensis) | fruitless | This paper | Figure 1—source data 1 | |
| Strain, strain background (Aedes aegypti, both sexes) | LVP-IB12 | BEI resources | MRA-735 | |
| Strain, strain background (Anopheles gambiae, both sexes) | G3 | Flaminia Catteruccia (Harvard University) | ||
| Strain, strain background (Culex quinquefasciatus, both sexes) | JHB | BEI resources | NR-43025 | |
| Strain, strain background (Wyeomyia smithii, both sexes) | PB | William Bradshaw and Christina Holzapfel (University of Oregon) | ||
| Strain, strain background (Toxorhynchites amboinensis, both sexes) | Laurence Zwiebel (Vanderbilt University) | |||
| Genetic reagent (Aedes aegypti, both sexes) | fruitless∆M | This paper | fruitless mutant strain; eggs available on request | |
| Genetic reagent (Aedes aegypti, both sexes) | fruitless∆M-tdTomato | This paper | fruitless mutant strain; eggs available on request | |
| Antibody | Mouse monoclonal anti-Apocrypta bakeri Orco antibody | Vanessa Ruta (The Rockefeller University) (PMID:30111839) | 15B2 | IF(1:50) |
| Antibody | Rabbit polyclonal anti-RFP antibody | Rockland | Cat# 600-401-379, RRID:AB_2209751 | IF(1:200) |
| Antibody | Mouse monoclonal anti-Brp antibody | DSHB | Cat# nc82, RRID:AB_2314866 | IF(1:5000) |
| Antibody | Goat polyclonal anti-mouse Alexa Fluor 488 antibody | Thermo Fisher | Cat# A-11001, RRID:AB_2534069 | IF(1:500) |
| Antibody | Goat polyclonal anti-mouse Alexa Fluor 555 Plus antibody | Thermo Fisher | Cat# A32732, RRID:AB_2633281 | IF(1:500) |
| Antibody | Goat polyclonal anti-mouse Alexa Fluor 647 polyclonal antibody | Thermo Fisher | Cat# A-21235, RRID:AB_2535804 | IF(1:500) |
| Recombinant DNA reagent | Fruitless∆-T2A-QF2 (plasmid) | This paper | Plasmid used to generatefruitless∆M strain | |
| Recombinant DNA reagent | Fruitless∆-T2A-CsChrimson-TdTomato (plasmid) | This paper | Plasmid used to generatefruitless∆M-tdTomato strain | |
| Commercial assay or kit | NEBuilder HiFi DNA Assembly | NEB | NEB:E5520S | |
| Commercial assay or kit | HiScribe Quick T7 kit | NEB | NEB:E2050S | |
| Peptide, recombinant protein | Cas9 | PNABio | PNABio:CP01-200 | |
| Sequence-based reagent | sgRNA_for | This paper | PCR primer for sgRNA | GAAATTAATACGACTCACTATAGCACCGAAGGTATGTTGAGGTTTTAGAGCTAGAAATAGC |
| Sequence-based reagent | sgRNA_rev | This paper | PCR primer for sgRNA | AAAAGCACCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATTTTAACTTGCTATTTCTAGCTCTAAAAC |
| Software, algorithm | STAR v 2.5.2a | RNAseq alignment | ||
| Software, algorithm | Trinity v 2013-03-25 | De novo assembly | ||
| Software, algorithm | Prism v8 | GraphPad | Prism 8 | statistics |
| Software, algorithm | MacVector v15.0.3 | MacVector | MacVector | Plasmid cloning |
| Other | DAPI stain | Sigma | D9542 | 1:10,000 |
| Other | Quattroport | This paper | https://github.com/VosshallLab/Basrur_Vosshall2020; Basrur, 2021; (copy archived atswh:1:rev:529b0ba393bac09207d298e73e425b452338e876) |