Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| VeroE6 cells (Cercopithecus aethiops) | Monkey kidney cell line | ATCC | CRL 1586TM | |
| Vero cells (Cercopithecus aethiops) | Monkey kidney cell line | WHO | RCB 10-87 | |
| Calu-3 (Homo sapiens) | Lung adenocarcinoma cell line | ATCC | HTB 55 | |
| SARS-CoV-2 BavPat1 | SARS-CoV-2 | Dr. Christian Drosten | European Virus Archive Global #026V-03883 | |
| SARS-CoV HKU39849 | SARS-CoV | Dr. Malik Peiris | N/A | |
| Airway tissue for organoids (Homo sapiens) | Airway organoids | This study | This study | |
| Aloxistatin | E64D | MedChemExpress | Cat# HY-100229 | |
| Camostat mesylate | Camostat | Sigma | Cat# SML0057 | |
| Hexadimethrine bromide | Polybrene | Sigma | 107689-10G | |
| Polyethylenimine linear | Polyethylenimine | Polysciences | Cat# 23966 | |
| Hygromycin B | Hygromycin B | Invitrogen | Cat# 10843555001 | |
| G418, Geneticin | Geneticin | Invitrogen | Cat# 10131035 | |
| Opti-MEM I (1×) + GlutaMAX | Opti-MEM I (1×) + GlutaMAX | Gibco | Cat# 51985-042 | |
| Advanced DMEM/F12 | Advanced DMEM/F12 | Thermo Fisher scientific | Cat# 12634-010 | |
| AO medium | AO medium | Sachs et al., 2019 | N/A | |
| Pneumacult ALI medium | Pneumacult ALI medium | Stemcell | Cat # 05001 | |
| TryplE | TryplE | Thermo Fisher Scientific | Cat# 12605010 | |
| Cultrex Basement Membrane Extract, Type 2 | Basement membrane extract | R&D Systems | Cat# 3533-005-02 | |
| 12 mm Transwell with 0.4 µm Pore Polyester Membrane Insert, Sterile | Transwell inserts | Corning | Cat# 3460 | |
| Collagen Type I, High concentration Rat tail | Collagen | Corning | Cat# 354249 | |
| 0.45 μm low protein binding filter | 0.45 μm low protein binding filter | Millipore | Cat# SLHV033RS | |
| Recombinant DNA reagent | pCMV-S (CUHK-W1) | Sino Biological | Cat# VG40150-G-N | Encoding S of isolate CUHK-W1; |
| Recombinant DNA reagent | pCAGGS-S (CUHK-W1) | This study | This study | Encoding S of isolate CUHK-W1; |
| Recombinant DNA reagent | pCAGGS-S (Wuhan-Hu-1) | This study | This study | Encoding S of isolate Wuhan-Hu-1 |
| Recombinant DNA reagent | pQXCIN | Clontech | Cat# 631516 | Retro-X Q vector set |
| Recombinant DNA reagent | pQXCIH | Clontech | Cat# 631516 | Retro-X Q vector set |
| Recombinant DNA reagent | pQXCIP-GFP1-10 | Addgene | Cat# 68715 | GFP1-10 |
| Recombinant DNA reagent | pCDNA-TMPRSS2-FLAG | Genscript | OHu13675D | Human TMPRSS2 cDNA |
| Recombinant DNA reagent | pCAGGS-β-Actin-7xGFP11-P2A-BFP | This study | This study | β-Actin-7xGFP11-P2A-BFP |
| Recombinant DNA reagent | pBS-gag-pol | Addgene | Cat# 35614 | gag-pol |
| Recombinant DNA reagent | pMD2.G | Addgene | Cat# 12259 | VSV-G |
| Recombinant DNA reagent | pVSV-eGFP-dG | Addgene | Cat# 31842 | VSV delta G genomic plasmid |
| Recombinant DNA reagent | pCAG-VSV-P | Addgene | Cat# 64088 | P protein |
| Recombinant DNA reagent | pCAG-VSV-L | Addgene | Cat# 64085 | L protein |
| Recombinant DNA reagent | pCAG-VSV-N | Addgene | Cat# 64087 | N protein |
| Recombinant DNA reagent | pCAGGS-T7Opt | Addgene | Cat# 65974 | T7 polymerase |
| Antibody | Mouse-anti-SARS-CoV NP (monoclonal) | Sino Biological | Cat# 40143-MM05 | IF (1:400) |
| Antibody | Rabbit-anti-SARS-CoV NP (polyclonal) | Sino Biological | Cat# 40143-T62 | IF (1:400) |
| Antibody | Goat anti-ACE2 (polyclonal) | R&D Systems | Cat# AF933 | IF (1:200) |
| Antibody | Mouse anti-TMPRSS2 (monoclonal) | Santa Cruz | Cat# sc-515727 | IF (1:200) |
| Antibody | Rabbit-anti-goat | Dako | Cat# P0160 | IF (1:400) |
| Antibody | Goat-anti-mouse | Dako | Cat# P0260 | IF (1:400) |
| Antibody | Mouse anti-VSV (monoclonal) | Absolute Antibody | Cat# EB0010 | Pseudoparticle production (1:50,000) |
| Antibody | Goat anti-rabbit IgG (H+L) Alexa Fluor Plus 488 | Invitrogen | Cat# A32731 | IF (1:400) |
| Antibody | Goat anti-mouse IgG (H+L) Alexa Fluor Plus 488 | Invitrogen | Cat# A11029 | IF (1:400) |
| Antibody | Goat anti-mouse IgG (H+L) Alexa Fluor Plus 594 | Invitrogen | Cat# A21125 | IF (1:400) |
| Antibody | Mouse anti-double stranded RNA IgG2A (monoclonal) | Scicons | J2 clone | IF (1:500) |
| Antibody | Mouse-anti-ZO1 IgG1 (monoclonal) | Invitrogen | Cat# 33-9100 | IF (1:200) |
| Antibody | Mouse-anti-CC10 IgG1 Alexa Fluor 594 (monoclonal) | Santa Cruz Biotechnology | Cat# sc-390313 AF594 | IF (1:100) |
| Antibody | Mouse-anti-AcTub IgG2A Alexa Fluor 488 (monoclonal) | Santa Cruz Biotechnology | Cat# sc-23950 AF488 | IF (1:100) |
| Antibody | Mouse anti-MUC5AC (monoclonal) | Invitrogen | Cat# MA5-12178 | IF (1:100) |
| Antibody | Mouse-anti-FOXJ1 IgG1 (monoclonal) | eBioscience | Cat# 14-9965-82 | IF (1:200) |
| Antibody | Rabbit anti-SARS-CoV S1 (polyclonal) | Sino Biological | Cat# 40150-T62 | WB (1:1000) |
| Antibody | Mouse anti-GAPDH (monoclonal) | Santa Cruz Biotechnology | Cat# sc-32233 | WB (1:1000) |
| Pierce Silver Stain for Mass Spectrometry | Silver stain | Pierce | Cat# 24600 | |
| TO-PRO-3 Iodide | TO-PRO3 | ThermoFisher | Cat# T3605 | |
| Phalloidin CruzFluor 647 Conjugate | Phalloidin | Santa Cruz | Cat# ec-363796 | |
| Hoechst 33342, Trihydrochloride, Trihydrate | Hoechst | ThermoFisher | Cat# H1399 | |
| 4× Laemmli Sample Buffer | Laemmli | Bio-Rad | Cat# 1610747 | |
| Odyssey CLx | Odyssey CLx | Licor | ||
| Amersham Typhoon Biomolecular Imager | Amersham Typhoon Biomolecular Image | GE Healthcare | ||
| Amersham Imager 600 | Amersham Imager 600 | GE Healthcare | ||
| LSM700 confocal microscope | LSM700 confocal microscope | Zeiss | ||
| Carl ZEISS Vert.A1 | Carl ZEISS Vert.A1 | Zeiss | ||
| ZEN software | ZEN | Zeiss | ||
| ImageQuant TL 8.2 | ImageQuant TL 8.2 | GE Healthcare | ||
| Studio Lite Ver 5.2 | Studio Lite Ver 5.2 | Licor | ||
| GraphPad PRISM 8 | GraphPad PRISM 8 | GraphPad | ||
| Adobe Illustrator | Illustrator | Adobe Inc |