Figure 5. Direct actions of TSLP on virus-specific CD8+ T cells during primary and recall LCMV infection.
(A) Schematic of LCMV infection experiment. 103 of WT (Thy1.1+/1.1+) and Crlf2-/- (Thy1.1+/1.2+) P14 naïve T cells were co-transferred into naïve WT C57BL/6 mice (Thy1.2+/1.2+) and on the following day the mice were infected i.p. with 2 × 106 pfu of LCMV Armstrong. Mice were analyzed at day 8 p.i. (B and C) or at a memory time point (>day 30 p.i.) (D and E). (B) TSLPR expression was assessed on LCMV-specific P14 CD8+ T cells in the tissues on day 8 p.i. (C) Proportion of WT and Crlf2-/- T cells at day 8 p.i. in the tissues (combined data from two independent experiments shown). (D) TSLPR expression on memory P14 T cells from a mouse seeded with P14 T cells and infected with LCMV Armstrong i.p. for >30 days. (E) The proportion of WT and Crlf2-/- P14 cells of transferred cells in the tissues at >30 days, a memory time point (combined data from two independent experiments shown). (F) Schematic of LCMV infection experiment. WT (Thy1.1+/1.1+) and Crlf2-/- (Thy1.1+/1.2+) P14 T cells were isolated from mice seeded with P14 T cells and infected with LCMV Armstrong i.p. for >30 days, and equal numbers of WT and Crlf2-/- cells (103 of each population) were co-transferred into naïve WT C57BL/6 mice (Thy1.2+/1.2+) on day −1. On the following day (day 0), the mice were infected with 2 × 106 pfu LCMV Armstrong i.p. Mice were analyzed at day 8 p.i. (G) Proportion of WT and Crlf2-/- T cells at day 8 p.i. with LCMV in the tissues, (combined data from two independent experiments shown). Data are mean ± SEM. ns = not significant; *p<0.05; **p<0.01; ****p<0.0001 using a two-tailed paired students t-test. Data shown are representative of at least two independent experiments.
Figure 5—figure supplement 1. TSLP protein expression during acute LCMV infection.
