Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Xenopus tropicalis) |
fPanx1 | GenBank | NM_001130256.1 | |
| Gene (Homo sapiens) |
hPANX1 | GenBank | NM_015368.4 | |
| Cell line (Spodoptera frugiperda) | Sf9 | Expression Systems, Davis, CA | Parent cell line: IPLB-Sf-21-AE, RRID:CVCL_0518 |
Clonal isolate derived from the parental cell line |
| Cell line (Homo sapiens) | HEK293T | ATCC | CRL-3216, RRID:CVCL_0063 | Negative for mycoplasma contamination at ATCC (obtained from Kodi Ravichandran lab) |
| Biological sample (Homo sapiens) | Caspase-3 | Kang et al. | ||
| Antibody | Anti-human/mouse/rat Pannexin-1 (rabbit monoclonal) | Cell Signaling Technology | D9M1C, RRID:AB_28000167 | 1:1000 dilution |
| Chemical compound, drug | Brain PC | Avanti Polar Lipids | 840053 | |
| Chemical compound, drug | Brain Total Lipid Extract | Avanti Polar Lipids | 131101 | |
| Chemical compound, drug | Brain PI(4,5)P2 | Avanti Polar Lipids | 840046 | |
| Chemical compound, drug | DPhPC | Avanti Polar Lipids | 860337 | |
| Chemical compound, drug | Sulforhodamine-B | Sigma Aldrich | S1402 | |
| Chemical compound, drug | Rhodamine B | Sigma Aldrich | 83689 | |
| Chemical compound, drug | Dextran, Tetramethylrhodamine,3000 MW, | ThermoFisher Scientific | D3307 | |
| Chemical compound, drug | Alexa 594 Carboxylic Acid, tris salt | ThermoFisher Scientific | A33082 | |
| Chemical compound, drug | Alexa 555 Carboxylic Acid, tris salt | ThermoFisher Scientific | A33080 | |
| Chemical compound, drug | ATTO 550 Phallodin | ATTO-TEC | AD 550–8 | |
| Chemical compound, drug | α-32P-ATP | PerkinElmer | BLU003H250UC | |
| Chemical compound, drug | 3H-Glutamate | PerkinElmer | NET490250UC | |
| Chemical compound, drug | 3H-Spermidine | PerkinElmer | NET522001MC | |
| Software, algorithm | LabView | Kiessling et al. | https://github.com/VolkerKirchheim/VK_TIRFsinglevesicleStep1 ; Narahari, 2021; (copy archived at 'swh:1:rev:79a55c09884d1fd3f4965ef1d1bf8f102711d828') |