Figure 2. Emx2 immunostaining in wild-type (WT) (myo6b:actb1-Gfp) neuromasts.

(A and D) Merged image of DAPI, (B and E) β-actin-GFP, and (C and F) anti-Emx2 staining of neuromasts. Emx2 immunoreactivity is not detected in dividing hair cell (HC) precursor (#1, arrowhead) or daughter HCs during Rock and Roll (#2). However, it is detectable in immature daughter HCs after Rock and Roll, in which the oriented hair bundle is not yet evident (#3, E, magenta arrows), as well as in mature HCs (#4). Both immature (#3) and mature HCs (#4, A→P) that are located in the anterior side of the neuromast are Emx2-positive. Insets in B and E are higher magnifications showing the hair bundle orientation pattern at the apex of HCs.