| Genetic reagent (Danio rerio) |
Tg(myo6b:actb1-GFP) |
PMC3426295 |
RRID:ZFIN_ZDB-GENO-120926-20
|
Kindt group (NIDCD, NIH) |
| Genetic reagent (Danio rerio) |
Tg(myo6b:emx2-p2a-nls-mCherry) |
PMC5388538 |
RRID:ZFIN_ZDB-GENO-170619-3
|
Wu group (NIDCD, NIH) |
| Genetic reagent (Danio rerio) |
Tg(myo6b:YFP-Tubulin) |
N.A |
N.A. |
Kindt group (NIDCD, NIH) |
| Genetic reagent (Danio rerio) |
Tg(myo6b: tdTomato) |
N.A |
RRID:ZFIN_ZDB-TGCONSTRCT-160316-7
|
Kindt group (NIDCD, NIH) |
| Genetic reagent (Danio rerio) |
emx2 ko |
PMC5388538 |
RRID:ZFIN_ZDB-ALT-170606-5
|
Kindt group (NIDCD, NIH) |
| Genetic reagent (Danio rerio) |
Emx2:GFP |
this paper |
N.A. |
Wu group (NIDCD, NIH) |
| Genetic reagent (Danio rerio) |
Tg(myo6b: RiboTag) |
PMC5939014 |
PRID:ZFIN_ZDB-ALT-180129–10 |
Kindt group (NIDCD, NIH) |
| Genetic reagent (Danio rerio) |
Tg(myo6b:actb1-GFP) |
PMC3426295 |
RRID:ZFIN_ZDB-GENO-120926-20
|
Kindt group (NIDCD, NIH) |
| Sequence-based reagent |
tracrRNA |
IDT |
Cat# 1072533 |
25 μM |
| Sequence-based reagent |
emx2_guide RNA 5’- atggaaagctgccgcgtcccagg-3’ |
IDT |
this paper |
50 μM |
| Sequence-based reagent |
tracrRNA |
IDT |
Cat# 1072533 |
25 μM |
| Recombinant DNA |
pT3TS-nls-zCas9-nls |
Addgene |
Cat# 46757 |
|
| Recombinant DNA |
pKHR4 |
PMC4806538 |
|
|
| Recombinant DNA |
pKHR4-LHA-emx2-RHA |
this paper |
this paper |
Wu group (NIDCD, NIH) |
| Antibodies |
Rabbit anti-Emx2 |
Trans Genic (fukuoka, japan) |
KO609 |
|
| Antibodies |
Mouse anti-GFP |
Thermo Fisher Scientific |
A11120 |
|
| Antibodies |
Rabbit anti-GFP |
Abcam |
Ab6556 |
|
| Antibodies |
Alexa Fluor 647 phalloidin |
Themo Fisher Scientific |
A22287 |
|
| Chemical compound, drug |
Nocodazole |
Sigma-Aldrich |
M1404 |
10 μg/ml |
| Recombinant proteins |
I-SceI |
NEB |
R0694S |
|
| Software and algorithms |
ImageJ |
NIH |
|
https://imagej.net/Fiji/Downloads |
| Software and algorithms |
ImageJ Fiji |
NIH |
|
https://imagej.net/Fiji/Downloads |
| Software and algorithms |
PoorMan3Dreg |
|
|
http://sybil.ece.ucsb.edu/pages/software.html |
| Software and algorithms |
MTrack J |
Meijering et al., 2012 |
|
https://imagescience.org/meijering/software/mtrackj/ |
| Software and algorithms |
Fluorender 2.16 |
University of Utah, SCI |
|
http://www.sci.utah.edu/software/fluorender.html |
