| Strain, strain background (Escherichia coli) |
BL21(DE3) |
Invitrogen |
|
T7 express, protein expression strain |
| Strain, strain background (Escherichia coli) |
BW25113 |
Datsenko and Wanner, 2000 |
|
rrnB3 ΔlacZ4787 ΔphoBR580 hsdR514 Δ(araBAD)567 Δ(rhaBAD)568 galU95 ΔendA9::FRT ΔuidA3::pir(wt) recA1 rph-1 |
| Strain, strain background (Escherichia coli) |
BW25113 △dolP
|
This paper |
|
BW25113 with dolP deleted |
| Strain, strain background (Escherichia coli) |
BW25113 △lpp,△rcsF
|
This paper |
|
BW25113 with lpp and rcsF deleted |
| Strain, strain background (Escherichia coli) |
BW25113 △lpp,△rcsF,△pgsA
|
This paper |
|
BW25113 with lpp, rcsF and pgsA genes deleted |
| Strain, strain background (Escherichia coli) |
BW25113 △clsA,△clsB,△clsC
|
This paper |
|
BW25113 with clsA, clsB and clsC genes deleted |
| genetic reagent (E. coli) |
KEIO library |
Datsenko and Wanner, 2000 |
|
Nonessential genes disrupted in E. coli BW25113 |
| Recombinant DNA reagent |
pKD4 |
Datsenko and Wanner, 2000 |
Plasmid |
Template for the amplification of a kanamycin resistance cassette flanked by FRT sites. |
| Recombinant DNA reagent |
pKD46 |
Datsenko and Wanner, 2000 |
Plasmid |
Temperature sensitive, low copy number plasmid encoding the Lambda RED recombinase genes under the control of an arabinose inducible promoter |
| Recombinant DNA reagent |
pCP20 |
Datsenko and Wanner, 2000 |
Plasmid |
Temperature sensitive plasmid encoding the FLP recombinase gene |
| Recombinant DNA reagent |
pET17b |
Novagen |
Plasmid |
T7 expression vector, AmpR |
| Recombinant DNA reagent |
pET17b dolP
|
This paper |
Plasmid |
pET17b with dolP cloned between NdeI and EcoRI |
| Recombinant DNA reagent |
pET17b dolP TM |
This paper |
Plasmid |
As described above with the dolP gene randomly disrupted by Transposon mutations |
| Recombinant DNA reagent |
pET17b dolP STm
|
This paper |
Plasmid |
pET17b with the S. typhimurium dolP gene cloned between NdeI and HindIII |
| Recombinant DNA reagent |
pET17b dolP H.i
|
This paper |
Plasmid |
pET17b encoding a codon optimised Haemophilus influenza dolP homolog |
| Recombinant DNA reagent |
pET17b dolP P.m
|
This paper |
Plasmid |
pET17b encoding a codon optimised Pasteurella multocida dolP homolog |
| Recombinant DNA reagent |
pET17b dolP N.m
|
This paper |
Plasmid |
pET17b encoding a codon optimised Neisseria meningitidis dolP homolog |
| Recombinant DNA reagent |
pET17b dolP V.c
|
This paper |
Plasmid |
pET17b encoding a codon optimised Vibrio cholera dolP homolog |
| Recombinant DNA reagent |
pET17b osmY
|
This paper |
Plasmid |
pET17b encoding a codon optimised E. coli K12 osmY
|
| Recombinant DNA reagent |
p(OM)OsmY |
This paper |
Plasmid |
pET17b encoding a codon optimised E. coli K12 osmY synthesised with the dolP signal sequence and acylation site in place of the osmY signal sequence |
| Recombinant DNA reagent |
pET20b |
Novagen |
Plasmid |
T7 expression vector, AmpR |
| Recombinant DNA reagent |
pET20b dolP
|
This paper |
Plasmid |
pET20b with dolP cloned between NdeI and EcoRI |
| Recombinant DNA reagent |
pET20b dolP PM |
This paper |
Plasmid |
pET20b with dolP cloned between NdeI and EcoRI with site-directed point mutations at various sites |
| Recombinant DNA reagent |
pET20b wbbL
|
This paper |
Plasmid |
pET20b with wbbL gene cloned between NdeI and HindIII |
| Recombinant DNA reagent |
pET20b dolP::mCherry
|
This paper |
Plasmid |
pET20b encoding dolP fused to a codon optimised mCherry gene via a C-terminal 11-codon flexible linker (GGSSLVPSSDP) |
| Recombinant DNA reagent |
pET26b dolPpelB::mCherry
|
This paper |
Plasmid |
pET26b dolP::mCherry with the dolP signal sequence replaced with that of pelB
|
| Recombinant DNA reagent |
pET20b dolPIM::mCherry
|
This paper |
Plasmid |
pET20b dolP::mCherry with codon 20 and 22 of dolP each mutated to aspartic acid |
| Recombinant DNA reagent |
pET20b dolPW127E::mCherry
|
This paper |
Plasmid |
pET20b dolP::mCherry with codon 127 mutated to glutamic acid |
